Journal: PLoS ONE

Article Title: Cyclosporine A Induces Apoptotic and Autophagic Cell Death in Rat Pituitary GH3 Cells

doi: 10.1371/journal.pone.0108981

Figure Lengend Snippet: GH3 cells were incubated in DMEM with and without 10% fetal bovine serum in the presence or absence of CsA (0 to 10 µM) for 10 h. Cell survival was determined using Cell Counting Kit-8 (A) and LC3 expression was determined by Western blotting (B) as described in . Immunofluorescence staining of LC3 and Lamp2 (C, D), DAPI staining (E), and DNA fragmentation (F) were captured as described in . E: a, with FBS; b, without FBS; c, 1.0 µM CsA; d, 2.5 µM CsA; e, 5.0 µM CsA; f, 10 µM CsA. Scale bars: C, 100 µm; D, 25 µm; E, 100 µm. *** p <0.001 vs. serum treatment. # p <0.05, ## p <0.01, ### p <0.001 vs. serum treatment.

Article Snippet: Rat pituitary GH3 cells, which originated from the growth hormone-producing tumor of the rat anterior pituitary and somatomammotroph phenotype, were purchased from ATCC (CCL-82.1) and were cultured in Dulbecco's modified Eagle's medium (DMEM, GibcoBRL, Gaithersburg, MD, USA) supplemented with or without 10% heat-inactivated fetal bovine serum (GibcoBRL) at 37°C in 5% CO 2 in a humidified atmosphere.

Techniques: Incubation, Cell Counting, Expressing, Western Blot, Immunofluorescence, Staining

Journal: PLoS ONE

Article Title: Cyclosporine A Induces Apoptotic and Autophagic Cell Death in Rat Pituitary GH3 Cells

doi: 10.1371/journal.pone.0108981

Figure Lengend Snippet: GH3 cells were incubated in DMEM with or without 10% fetal bovine serum in the presence or absence of CsA (0 to 10 µM) for 10 h. p53 expression (A) was determined by Western blotting and the relative amount (B) was calculated as described in the . * p <0.05, ** p <0.01 vs. serum treatment. # p <0.05, ### p <0.001 vs. no serum treatment.

Article Snippet: Rat pituitary GH3 cells, which originated from the growth hormone-producing tumor of the rat anterior pituitary and somatomammotroph phenotype, were purchased from ATCC (CCL-82.1) and were cultured in Dulbecco's modified Eagle's medium (DMEM, GibcoBRL, Gaithersburg, MD, USA) supplemented with or without 10% heat-inactivated fetal bovine serum (GibcoBRL) at 37°C in 5% CO 2 in a humidified atmosphere.

Techniques: Incubation, Expressing, Western Blot

Journal: PLoS ONE

Article Title: Cyclosporine A Induces Apoptotic and Autophagic Cell Death in Rat Pituitary GH3 Cells

doi: 10.1371/journal.pone.0108981

Figure Lengend Snippet: GH3 cells were incubated in DMEM with or without 10% fetal bovine serum in the presence or absence of CsA (0 to 10 µM) for 10 h. Levels of p-ERK for autophagy (A) and apoptosis (C) were determined by Western blotting and the relative amount of p-ERK for autophagy (B) and apoptosis (D) was calculated as described in the . *** p <0.01 vs. serum treatment. ## p <0.01, ### p <0.001 vs. no serum treatment.

Article Snippet: Rat pituitary GH3 cells, which originated from the growth hormone-producing tumor of the rat anterior pituitary and somatomammotroph phenotype, were purchased from ATCC (CCL-82.1) and were cultured in Dulbecco's modified Eagle's medium (DMEM, GibcoBRL, Gaithersburg, MD, USA) supplemented with or without 10% heat-inactivated fetal bovine serum (GibcoBRL) at 37°C in 5% CO 2 in a humidified atmosphere.

Techniques: Incubation, Western Blot

Journal: PLoS ONE

Article Title: Cyclosporine A Induces Apoptotic and Autophagic Cell Death in Rat Pituitary GH3 Cells

doi: 10.1371/journal.pone.0108981

Figure Lengend Snippet: GH3 cells were incubated in DMEM with or without 10% fetal bovine serum in the presence or absence of CsA (0 to 10 µM) for 10 h. Levels of Bax and Bcl-2 for autophagy (A) and apoptosis (E) were determined by Western blotting and the relative amount of Bax and Bcl-2 for autophagy (B, C) and apoptosis (F, G) was calculated as described in the . Bcl-2 was imaged on an OLYMPUS DP controller and manager using an inverted microscope (D). * p <0.05, ** p <0.01, *** p <0.001 vs. serum treatment. # p <0.05, ### p <0.001 vs. no serum treatment. Scale bar is 100 µm.

Article Snippet: Rat pituitary GH3 cells, which originated from the growth hormone-producing tumor of the rat anterior pituitary and somatomammotroph phenotype, were purchased from ATCC (CCL-82.1) and were cultured in Dulbecco's modified Eagle's medium (DMEM, GibcoBRL, Gaithersburg, MD, USA) supplemented with or without 10% heat-inactivated fetal bovine serum (GibcoBRL) at 37°C in 5% CO 2 in a humidified atmosphere.

Techniques: Incubation, Western Blot, Inverted Microscopy

Journal: PLoS ONE

Article Title: Cyclosporine A Induces Apoptotic and Autophagic Cell Death in Rat Pituitary GH3 Cells

doi: 10.1371/journal.pone.0108981

Figure Lengend Snippet: GH3 cells were incubated in DMEM with or without 10% fetal bovine serum in the presence or absence of CsA (0 to 10 µM) for 10 h. Cu/Zn- and Mn-SOD levels for autophagy (A) and apoptosis (D) were determined by Western blotting and the relative amount for autophagy (B, C) and apoptosis (E, F) was calculated as described in the . * p <0.05, *** p <0.001 vs. serum treatment. ### p <0.001 vs. no serum treatment.

Article Snippet: Rat pituitary GH3 cells, which originated from the growth hormone-producing tumor of the rat anterior pituitary and somatomammotroph phenotype, were purchased from ATCC (CCL-82.1) and were cultured in Dulbecco's modified Eagle's medium (DMEM, GibcoBRL, Gaithersburg, MD, USA) supplemented with or without 10% heat-inactivated fetal bovine serum (GibcoBRL) at 37°C in 5% CO 2 in a humidified atmosphere.

Techniques: Incubation, Western Blot

Journal: PLoS ONE

Article Title: Cyclosporine A Induces Apoptotic and Autophagic Cell Death in Rat Pituitary GH3 Cells

doi: 10.1371/journal.pone.0108981

Figure Lengend Snippet: GH3 cells were incubated in DMEM with or without 10% fetal bovine serum in the presence or absence of CsA (0 to 10 µM) for 10 h. Calbindin-D9k levels for autophagy (A) and apoptosis (C) were determined by Western blotting and the relative amount of calbindin-D9k for autophagy (B) and apoptosis (D) was calculated as described in the . * p <0.05, ** p <0.01, *** p <0.001 vs. serum treatment. ## p <0.01, ### p <0.001 vs. no serum treatment.

Article Snippet: Rat pituitary GH3 cells, which originated from the growth hormone-producing tumor of the rat anterior pituitary and somatomammotroph phenotype, were purchased from ATCC (CCL-82.1) and were cultured in Dulbecco's modified Eagle's medium (DMEM, GibcoBRL, Gaithersburg, MD, USA) supplemented with or without 10% heat-inactivated fetal bovine serum (GibcoBRL) at 37°C in 5% CO 2 in a humidified atmosphere.

Techniques: Incubation, Western Blot